I am super duper happy!

I am so happy now... .super duper happy!!! yeah! yeah!! I had been having trouble cloning my gene (id:0963) into the pET101-DTOPO plasmid... and had previously failed in all my countless attempts to clone it! And yesterday, I prepared fresh PCR product from my gene0963 and cloned it with the cloning kit (FOC replacement since the previously might inherently be problematic) from Invitrogen. And I screened 7 colonies to see if my gene is inserted into the plasmid...

And I was feeling extremely nervous when I'm about to see the result in the gel-doc room... Will there be any positive result that i hope to see? If there is still no good result, what will become of me!? And the moment when I see that 2 out of the 7 bacteria colonies has got my gene0963 insert, I almost jumped for joy! You wouldn't have imagine how happy I am! After all these weeks of not being able to get it in, today I finally manage to put the gene into the plasmid! Despite another gene (id:0695) of mine apparently didn't managed to be cloned, still I couldn't be any more happier (since this is only the first attempt to clone 0695).

And as I was walking back from the gel-doc room to the lab, I couldn't help but keep on smiling at myself... Luckily it's a Saturday and there isn't a single soul along the way, else they might think I'm nuts! Hahaha.. but I'm very happy! yeah! yeah! And today is the 4years 6 months anniversary of my relationship with my dearly Limin. Indeed, as she said, the anniversary might have brought me luck!